Research Article
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In vitro Biological Activities of Different Extracts from Alcea dissecta

Year 2022, Volume: 12 Issue: 1, 53 - 60, 30.03.2022
https://doi.org/10.33808/clinexphealthsci.787845

Abstract

Objective: Alcea genus belongs to Malvaceae family and this genus is represented by 85 taxa in the world and 21 taxa in the Flora of Turkey.
The flowers of Alcea genus contain plenty of mucilage and are used for the treatment of respiratory diseases such as asthma and cough.
Alcea dissecta is known as ‘Govik, Hiro, Hero’ in Turkey, and the flowers and leaves of this species have been used in the treatment of asthma,
injury, colds, and gastrointestinal diseases in Turkey. To the best of our knowledge, there is no report on the effect of extraction methods on
the biological activity of this plant. In addition, although this species is being used as a medical plant, there is no study of the antioxidant, antiurease,
esterase, and anticholinesterase activity of the plant. Therefore, the aim of this study was to evaluate in vitro antioxidant, anti-urease,
esterase, anticholinesterase activities of Alcea dissecta using a variety of extracts.
Methods: The antioxidant activities of different extracts were examined by DPPH, ABTS, FRAP, and CUPRAC methods. The total phenolic
compounds contained in the extracts were determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease and anticholinesterase
activities of different extracts were evaluated by indophenol and Ellman methods respectively. In addition, esterase activities of plant extracts
were determined.
Results: In the present study, ethanol:water (1:1, v/v) and chloroform extracts obtained maceration method showed stronger DPPH and ABTS
radical scavenging activity than other extracts. The chloroform extract obtained Soxhlet method was found to have higher FRAP and CUPRAC
values than other extracts. It was also found that the ethanol extract obtained maceration method showed the most potent anti-urease and
anticholinesterase activity. According to the results, the strongest inhibitory effect on both hCA I and II isoenzymes was shown by the petroleum
ether extract obtained Soxhlet method.
Conclusion: As a result, it was determined that different plant extracts have antioxidant, anti-urease, esterase, anticholinesterase activities. In
addition, the data obtained from this study will shed light on future research on the biological activities of this species.

References

  • Bibi F. Diversity of antagonistic bacteria isolated from medicinal plant Peganum harmala L. Saudi J Biol Sci 2017; 24: 1288-1293.
  • Azwanida NN. A Review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4(3): 1-6.
  • Amin M, Anwar F, Naz F, Mehmood T, Saari N. Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 2013; 18: 2135-2149.
  • Modolo LV, Souza AX, Horta LP, Araujo DP, Fátima A. An overview on the potential of natural products as ureases inhibitors: A review. J Adv Res 2015; 6(1): 35-44.
  • Wang YC. Medicinal plant activity on Helicobacter pylori related diseases. World J Gastroenterol 2014; 20(30): 10368-10382.
  • Levent S, Çevik UA, Sağlık BN, Özkay Y, Can ÖD, Özkay ÜD, Uçucu Ü. Anticholinesterase activity screening of some novel dithiocarbamate derivatives including piperidine and piperazine moieties. Phosphorus Sulfur Silicon Relat Elem 2017; 192(4): 469-474.
  • Neagu E, Radu GL, Albu C, Paun G. Antioxidant activity, acetylcholinesterase and tyrosinase inhibitory potential of Pulmonaria officinalis and Centarium umbellatum extracts. Saudi J Biol Sci 2018; 25: 578-585.
  • Arslan T, Çelik G, Celik H, Şentürk M, Yaylı N, Ekinci D. Synthesis and Biological Evaluation of Novel Bischalcone Derivatives as Carbonic Anhydrase Inhibitors. Arch Pharm (Weinheim) 2016; 349: 741-748.
  • Kuzu M, Özkaya A, Şahin Z, Dağ Ü, Comakli V, Demirdağ R. In Vivo Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme. Turk J Pharm Sci 2017; 14(1): 9-12.
  • Türkoğlu EA, Şentürk M, Supuran CT, Ekinci D. Carbonic anhydrase inhibitory properties of some uracil derivatives. J Enzyme Inhib Med Chem 2017; 32: 74-77.
  • Supuran CT. Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 2008; 7(2): 168-181.
  • Arslan T, Türkoğlu EA, Şentürk M, Supuran CT. Synthesis and carbonic anhydrase inhibitory properties of novel chalcone substituted benzenesulfonamides. Bioorg Med Chem Lett 2016; 26: 5867-5870.
  • Orhan F, Şentürk M, Supuran CT. Interaction of anions with a newly characterized alpha carbonic anhydrase from Halomonas sp. J Enzyme Inhib Med Chem 2016; 31: 1119-1123.
  • Altemini A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants 2017; 6: 42.
  • The Plant List, A working list of all plant species. TPL (serial online) 2013 (cited 2021 October 15). Available from: URL: http://www.theplantlist.org/tpl1.1/search?q=alcea.
  • Güner A, Aslan S, Ekim T, Vural M, Babaç MT. (edlr.). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını; İstanbul; 2012.
  • Uzunhisarcikli ME, Vural M. The taxonomic revision of Alcea and Althaea (Malvaceae) in Turkey. Turk J Bot 2012; 36(6): 603-636.
  • Khidyrova NK, Rakhmatova MZh, Kukina TP, Shakhidoyatov RKh, Shakhidoyatov KhM. Polyprenols and triterpenoids from leaves of Alcea nudiflora. Chem Nat Compd 2012; 48(2): 180-184.
  • Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Soc Behav Sci 2011; 19: 756-777.
  • Tuzlacı E, Doğan A. Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharm J 2010; 14: 136-143.
  • Bulut G, Korkmaz A, Tuzlacı E. The ethnobotanical notes from Nizip (Gaziantep-Turkey). Istanbul J Pharm 2017; 47(2): 57-62.
  • Samatha T, Shyamsundarachary R, Srinivas P, Swamy NR. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian J Pharm Clin Res 2012; 5(4): 177-179.
  • Wei F, Jinglou C, Yaling C, Yongfang L, Liming C, Lei P, Zhou D, Liang X, Ruan J. Antioxidant, free radical scavenging, antiinflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch.et Sav.) Ching. J Ethnopharmacol 2010; 130: 521-528.
  • Re R, Pellegrını N, Proteggente A, Pannala A, Yang M, Evans CR. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26: 1231-1237.
  • Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 1996; 239(1): 70-76.
  • Apak R, Güclü K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agric Food Chem 2004; 52: 7970-7981.
  • Taşkın D, Taşkın T, Rayaman E. Phenolic composition and biological properties of Achillea nobilis L. subsp. neilreichii (Kerner) Formanek. Ind Crops Prod 2018; 111: 555-562.
  • Ellman GL, Courtney KD, Andress V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7(2): 88-95.
  • Verpoorte JA, Mehta S, Edsall JT. Esterase activities of human carbonic anhydrases B and C. J Biol Chem 1967; 242: 4221-4229.
  • Demirdag R, Comakli V, Kuzu M, Yerlikaya E, Şentürk M. Purification and Characterization of Carbonic Anhydrase from Ağrı Balık Lake Trout Gill (Salmo trutta labrax) and Effects of Sulfonamides on Enzyme Activity. J Biochem Mol Toxicol 2015; 29(3): 123-128.
  • Balaydin HT, Durdagi S, Ekinci D, Şentürk M, Göksu S, Menzek A. Inhibition of human carbonic anhydrase isozymes I, II and VI with a series of bisphenol, methoxy and bromophenol compounds. J Enzyme Inhib Med Chem 2012; 27: 467-475.
  • Alhage J, Elbitar H. In vitro screening for antioxidant and antimicrobial properties of three lebanese medicinal plants crude extracts. Pharmacognosy Research 2019; 11(2): 127-133.
  • Yaglıoglu AS, Eser F, Tekin S, Onal A. Antiproliferative activities of several plant extracts from Turkey on rat brain tumor and human cervix carcinoma cell lines. Front Life Sci 2016; 9(1): 69-74.
  • Mombeini T, Pourbaide HG, Kamalinejad M, Mazloumi S, Dehpour AR. Anxiolytic-like and sedative effects of Alcea aucheri (Boiss.) Alef. flower extract in the laboratory rat. Iran J Pharm Res 2017; 16(4): 1495-1508.
  • Zakizadeh M, Nabavi SF, Nabavi SM & Ebrahimzadeh MA. In vitro antioxidant activity of flower, seed and leaves of Alcea hyrcana Grossh. Eur Rev Med Pharmacol Sci 2011; 15: 406-412.
  • Kırca A, Arslan D. Antioxidant capacity and total phenolic content of selected plants from Turkey, Int J Food Sci Tech 2008; 43: 2038-2046.
Year 2022, Volume: 12 Issue: 1, 53 - 60, 30.03.2022
https://doi.org/10.33808/clinexphealthsci.787845

Abstract

References

  • Bibi F. Diversity of antagonistic bacteria isolated from medicinal plant Peganum harmala L. Saudi J Biol Sci 2017; 24: 1288-1293.
  • Azwanida NN. A Review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4(3): 1-6.
  • Amin M, Anwar F, Naz F, Mehmood T, Saari N. Anti-Helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules 2013; 18: 2135-2149.
  • Modolo LV, Souza AX, Horta LP, Araujo DP, Fátima A. An overview on the potential of natural products as ureases inhibitors: A review. J Adv Res 2015; 6(1): 35-44.
  • Wang YC. Medicinal plant activity on Helicobacter pylori related diseases. World J Gastroenterol 2014; 20(30): 10368-10382.
  • Levent S, Çevik UA, Sağlık BN, Özkay Y, Can ÖD, Özkay ÜD, Uçucu Ü. Anticholinesterase activity screening of some novel dithiocarbamate derivatives including piperidine and piperazine moieties. Phosphorus Sulfur Silicon Relat Elem 2017; 192(4): 469-474.
  • Neagu E, Radu GL, Albu C, Paun G. Antioxidant activity, acetylcholinesterase and tyrosinase inhibitory potential of Pulmonaria officinalis and Centarium umbellatum extracts. Saudi J Biol Sci 2018; 25: 578-585.
  • Arslan T, Çelik G, Celik H, Şentürk M, Yaylı N, Ekinci D. Synthesis and Biological Evaluation of Novel Bischalcone Derivatives as Carbonic Anhydrase Inhibitors. Arch Pharm (Weinheim) 2016; 349: 741-748.
  • Kuzu M, Özkaya A, Şahin Z, Dağ Ü, Comakli V, Demirdağ R. In Vivo Effects of Naringenin and Lead on Rat Erythrocyte Carbonic Anhydrase Enzyme. Turk J Pharm Sci 2017; 14(1): 9-12.
  • Türkoğlu EA, Şentürk M, Supuran CT, Ekinci D. Carbonic anhydrase inhibitory properties of some uracil derivatives. J Enzyme Inhib Med Chem 2017; 32: 74-77.
  • Supuran CT. Carbonic anhydrases: novel therapeutic applications for inhibitors and activators. Nat Rev Drug Discov 2008; 7(2): 168-181.
  • Arslan T, Türkoğlu EA, Şentürk M, Supuran CT. Synthesis and carbonic anhydrase inhibitory properties of novel chalcone substituted benzenesulfonamides. Bioorg Med Chem Lett 2016; 26: 5867-5870.
  • Orhan F, Şentürk M, Supuran CT. Interaction of anions with a newly characterized alpha carbonic anhydrase from Halomonas sp. J Enzyme Inhib Med Chem 2016; 31: 1119-1123.
  • Altemini A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA. Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants 2017; 6: 42.
  • The Plant List, A working list of all plant species. TPL (serial online) 2013 (cited 2021 October 15). Available from: URL: http://www.theplantlist.org/tpl1.1/search?q=alcea.
  • Güner A, Aslan S, Ekim T, Vural M, Babaç MT. (edlr.). Türkiye Bitkileri Listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını; İstanbul; 2012.
  • Uzunhisarcikli ME, Vural M. The taxonomic revision of Alcea and Althaea (Malvaceae) in Turkey. Turk J Bot 2012; 36(6): 603-636.
  • Khidyrova NK, Rakhmatova MZh, Kukina TP, Shakhidoyatov RKh, Shakhidoyatov KhM. Polyprenols and triterpenoids from leaves of Alcea nudiflora. Chem Nat Compd 2012; 48(2): 180-184.
  • Altundag E, Ozturk M. Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Soc Behav Sci 2011; 19: 756-777.
  • Tuzlacı E, Doğan A. Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharm J 2010; 14: 136-143.
  • Bulut G, Korkmaz A, Tuzlacı E. The ethnobotanical notes from Nizip (Gaziantep-Turkey). Istanbul J Pharm 2017; 47(2): 57-62.
  • Samatha T, Shyamsundarachary R, Srinivas P, Swamy NR. Quantification of total phenolic and total flavonoid contents in extracts of Oroxylum indicum L. Kurz. Asian J Pharm Clin Res 2012; 5(4): 177-179.
  • Wei F, Jinglou C, Yaling C, Yongfang L, Liming C, Lei P, Zhou D, Liang X, Ruan J. Antioxidant, free radical scavenging, antiinflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica (Franch.et Sav.) Ching. J Ethnopharmacol 2010; 130: 521-528.
  • Re R, Pellegrını N, Proteggente A, Pannala A, Yang M, Evans CR. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26: 1231-1237.
  • Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem 1996; 239(1): 70-76.
  • Apak R, Güclü K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agric Food Chem 2004; 52: 7970-7981.
  • Taşkın D, Taşkın T, Rayaman E. Phenolic composition and biological properties of Achillea nobilis L. subsp. neilreichii (Kerner) Formanek. Ind Crops Prod 2018; 111: 555-562.
  • Ellman GL, Courtney KD, Andress V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7(2): 88-95.
  • Verpoorte JA, Mehta S, Edsall JT. Esterase activities of human carbonic anhydrases B and C. J Biol Chem 1967; 242: 4221-4229.
  • Demirdag R, Comakli V, Kuzu M, Yerlikaya E, Şentürk M. Purification and Characterization of Carbonic Anhydrase from Ağrı Balık Lake Trout Gill (Salmo trutta labrax) and Effects of Sulfonamides on Enzyme Activity. J Biochem Mol Toxicol 2015; 29(3): 123-128.
  • Balaydin HT, Durdagi S, Ekinci D, Şentürk M, Göksu S, Menzek A. Inhibition of human carbonic anhydrase isozymes I, II and VI with a series of bisphenol, methoxy and bromophenol compounds. J Enzyme Inhib Med Chem 2012; 27: 467-475.
  • Alhage J, Elbitar H. In vitro screening for antioxidant and antimicrobial properties of three lebanese medicinal plants crude extracts. Pharmacognosy Research 2019; 11(2): 127-133.
  • Yaglıoglu AS, Eser F, Tekin S, Onal A. Antiproliferative activities of several plant extracts from Turkey on rat brain tumor and human cervix carcinoma cell lines. Front Life Sci 2016; 9(1): 69-74.
  • Mombeini T, Pourbaide HG, Kamalinejad M, Mazloumi S, Dehpour AR. Anxiolytic-like and sedative effects of Alcea aucheri (Boiss.) Alef. flower extract in the laboratory rat. Iran J Pharm Res 2017; 16(4): 1495-1508.
  • Zakizadeh M, Nabavi SF, Nabavi SM & Ebrahimzadeh MA. In vitro antioxidant activity of flower, seed and leaves of Alcea hyrcana Grossh. Eur Rev Med Pharmacol Sci 2011; 15: 406-412.
  • Kırca A, Arslan D. Antioxidant capacity and total phenolic content of selected plants from Turkey, Int J Food Sci Tech 2008; 43: 2038-2046.
There are 36 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Turgut Taşkın 0000-0001-8475-6478

Dilay Kahvecioğlu This is me 0000-0001-8748-7269

Emir Alper Türkoğlu 0000-0001-7850-6456

Ahmet Doğan 0000-0003-0603-5100

Müslüm Kuzu 0000-0002-1375-7673

Publication Date March 30, 2022
Submission Date September 10, 2020
Published in Issue Year 2022 Volume: 12 Issue: 1

Cite

APA Taşkın, T., Kahvecioğlu, D., Türkoğlu, E. A., Doğan, A., et al. (2022). In vitro Biological Activities of Different Extracts from Alcea dissecta. Clinical and Experimental Health Sciences, 12(1), 53-60. https://doi.org/10.33808/clinexphealthsci.787845
AMA Taşkın T, Kahvecioğlu D, Türkoğlu EA, Doğan A, Kuzu M. In vitro Biological Activities of Different Extracts from Alcea dissecta. Clinical and Experimental Health Sciences. March 2022;12(1):53-60. doi:10.33808/clinexphealthsci.787845
Chicago Taşkın, Turgut, Dilay Kahvecioğlu, Emir Alper Türkoğlu, Ahmet Doğan, and Müslüm Kuzu. “In Vitro Biological Activities of Different Extracts from Alcea Dissecta”. Clinical and Experimental Health Sciences 12, no. 1 (March 2022): 53-60. https://doi.org/10.33808/clinexphealthsci.787845.
EndNote Taşkın T, Kahvecioğlu D, Türkoğlu EA, Doğan A, Kuzu M (March 1, 2022) In vitro Biological Activities of Different Extracts from Alcea dissecta. Clinical and Experimental Health Sciences 12 1 53–60.
IEEE T. Taşkın, D. Kahvecioğlu, E. A. Türkoğlu, A. Doğan, and M. Kuzu, “In vitro Biological Activities of Different Extracts from Alcea dissecta”, Clinical and Experimental Health Sciences, vol. 12, no. 1, pp. 53–60, 2022, doi: 10.33808/clinexphealthsci.787845.
ISNAD Taşkın, Turgut et al. “In Vitro Biological Activities of Different Extracts from Alcea Dissecta”. Clinical and Experimental Health Sciences 12/1 (March 2022), 53-60. https://doi.org/10.33808/clinexphealthsci.787845.
JAMA Taşkın T, Kahvecioğlu D, Türkoğlu EA, Doğan A, Kuzu M. In vitro Biological Activities of Different Extracts from Alcea dissecta. Clinical and Experimental Health Sciences. 2022;12:53–60.
MLA Taşkın, Turgut et al. “In Vitro Biological Activities of Different Extracts from Alcea Dissecta”. Clinical and Experimental Health Sciences, vol. 12, no. 1, 2022, pp. 53-60, doi:10.33808/clinexphealthsci.787845.
Vancouver Taşkın T, Kahvecioğlu D, Türkoğlu EA, Doğan A, Kuzu M. In vitro Biological Activities of Different Extracts from Alcea dissecta. Clinical and Experimental Health Sciences. 2022;12(1):53-60.

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