BibTex RIS Cite

Determination of resistance to <em>Bean common mosaıc virus</em> (BCMV) and <em>Bean common mosaic necrosis virus</em> (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods

Year 2013, Volume: 53 Issue: 2, 101 - 113, 03.07.2013

Abstract

In 2007, a total of five bean cultivars, two of which are commercial cultivars, and one breeding line were evaluated for presence of known resistance genes and resistance levels to BCMV and BCMNV using qualitative, quantitative and molecular methods. Five plants of each cultivars and breeding line were inoculated with NL-3 strain of BCMNV and NL-4 strain of BCMV, and all plants were evaluated for virus symptoms and vigor. Additionally, plants were tested by DAS-ELISA to evaluate the virus titer on non-inoculated trifoliate leaves quantitatively. Also, two SCAR markers, SW-13 linked to the dominant I gene and SBD-5 for the recessive gene bc-12 were used to screen these genes in those cultivars by PCR. Results showed that cultivars Zulbiye and Ozeren Seker and 4F-3260 breeding line posses the dominant I gene which is responsible for resistance to BCMV, and Akdag has the recessive gene bc-22 which confers resistance to all known strains of BCMNV and BCMV except NL-4. Cultivars Ozeren Seker and Ozeren Barbunya occurred to possess the recessive gene bc-12 which is responsible for resistance to four strains of BCMV (NL-1, NL-7, US-2, NL-2) and NL-8 strain of BCMNV.

References

  • Ali M.A. 1950. Genetics of resistance to the common bean mosaic virus in the bean (Phaseolus vulgaris L.). Phytopathology 40:69-79.
  • Clark M.R. and Adams A. M. 1977. Characteristics of the microplate method of EnzymeLinked Immunosorbent Assay for the detection of plant viruses. J.General Virology, 34:475-483.
  • Collmer C.W. Marston M.F. Taylor J.C. Jahn M.M. 2000. The I gene of bean: a dosagedependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus. Molecular. Plant-Microbe Interactions. 13:1266–1270.
  • Cooper L.I. and Jones A.T. 1983. Responses of plants to viruses: Proposals for the use of terms. Phytopathology 73, 127-128.
  • Coyne D.P. Steadman J.R. Godoy-Lutz G. Gilbertson R. Arnaud-Santana E. Beaver J.S. and Myers J.R. 2003. Contributions of the Bean/Cowpea CRSP to management of bean diseases. Field Crops Research, 82: 155-168.
  • Drijfhout E. 1978. Genetic interaction between Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. Doctoral thesis. Wageningen Agric. Res. Rep. 872.
  • Drijfhout, E. Silbernagel M.J. and Burke D.W. 1978. Differentiation of strains of Bean common mosaic virus. Neth. J. Plant Pathol., 84: 13-26.
  • Drijfhout E. 1994. Bean common mosaic virus (In: compendium of bean diseases, Second Edition. Ed: R. Hall). APS Press, St. Paul, Minnesota, USA.
  • Gilbertson R.L. Guzman P. Rojas M. Crnov R. and Mkandawıre A. 2001. Detection of bean-infecting viruses in California with an emphasis on the CRSP-facilitated work. Bean/Cowpea Collaborative Research Support Program-East Africa Proceedings: Bean Seed Workshop Arusha, Tanzania. pp. 4.
  • Haley S.D. Afanador L. and Kelly J.D. 1994a. Identificaton and application of random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathology 84, 157-160.
  • Haley S.D. Afanador L.K. and Kelly J.D. 1994b. Selection for monogenic resistance traits with coupling and repulsion phase RAPD markers. Crop Science 34, 1061-1066.
  • Johnson W.C. and Gepts P. 1994. Two molecular markers linked to bc-3. Bean Improvement Cooperative Annual Report 37, 206-207.
  • Johnson W.C. Guzman P. Mandala D. Mkandawıre A.B.C. Temple S. Gilbertson R.L. and Gepts P. 1997. Moleculer tagging of the bc-3 gene for introgression into Andean common bean. Crop Sci. 37: 248-254.
  • Kelly J.D. 1997. A review of varietal response to Bean common mosaic potyvirus in Phaseoulus vulgaris. Plant Var. Seeds, 10: 1-6.
  • Larsen R.C. Miklas P.N. Druffel K.L. and Wyatt S.D. 2005. NL3-K strain is a stable and naturally occuring interspesific recombinant derived from bean common mosaic necrosis virus and bean common mosaic virus. Phytopathology. Vol: 95. No. 9 1037-1042.
  • Melotto M. Afanador L. and Kelly J.D. 1996. Development of SCAR marker linked to the I gene in common bean. Genome 39, 1216-1219.
  • Miklas P.N. Afanador L. and Kelly J.D. 1996. Recombination facilitated RAPD marker assisted selection for disease resistance in common bean. Crop Science 36, 86-90.
  • Miklas P. N. Larsen R.C. Riley R. and Kelly J. D. 2000. Potential marker-assisted selection for bc-1² resistance to Bean common mosaic potyvirus in common bean. Euphytica, 116 (3): 211-219.
  • Mukeshimena G. Paneda A. Rodriguez-Suarez C. Ferreria J.J. Giraldez R. and Kelly J.D. 200 Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean. Euphytica. 144: 291-299. Myers J.R. Strausbaugh C.A. Forster R.L. and Mcclean P.E. 1996. Resistance and tolerance to bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in bean. Bean Improvement Cooperative Annual Report 39, 94-95.
  • Naderpour M. Lund O.S. Larsen R. Johansen E. 2010. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eIF4E allele. Molecualr Plant Pathology 11:255-263.
  • Ogliari J.B. and Castano M. 1992. Identification of Resistant Germplasm to the Bean common mosaic virus-BCMV. Pesquisa Agropecuaria Brasileira 27(7) :1043-1047. Sengooba T.N. Spence N.F. Walket D.G.A. Allen D.J. and Femi-Lana A. 1997. The occurrence of bean common mosaic necrosis virus in wild and forage legumes in Uganda. Plant Pathology. 46, 95-103.
  • Silbernagel M.J. 1995. Bean germplasm releases. Annu.Rep. Bean Improv. Coop. 38: 177.

Bazı fasulye genotiplerinin Bean common mosaic virus (BCMV) ve Bean common mosaic necrosis virus (BCMNV)’a dayanıklılık durumlarının kalitatif, kantitatif ve moleküler yöntemlerle belirlenmesi

Year 2013, Volume: 53 Issue: 2, 101 - 113, 03.07.2013

Abstract

2007 yılında ikisi tescilli olmak üzere beş fasulye çeşidi ve bir ıslah hattının BCMV ve BCMNV'ye karşı dayanıklılık düzeylerinin ve sahip oldukları dayanıklılık genlerinin kalitatif, kantitatif ve genetik olarak belirlenmesine çalışılmıştır. Bu amaçla her çeşitten beş bitki, BCMNV NL-3 ırkı ve BCMV NL-4 ırkı ile inokule edilerek çeşitlerin dayanıklılık düzeyleri simptomatolojik olarak incelenmiştir. Buna ilave olarak, DAS-ELISA yöntemi ile çeşitlerdeki virüs enfeksiyon düzeyi kantitatif olarak da ortaya konmuştur. Ayrıca, dominant I genine spesifik SW-13 ve resesif bc-12 genine spesifik SBD-5 SCAR markörleri ile PCR yöntemi uygulanarak çeşitlerin söz konusu genleri bulundurma durumu moleküler olarak belirlenmiştir. Zülbiye ve Özeren Şeker çeşitlerinin ve 4F-3260 ıslah hattının BCMV'ye karşı dayanıklılıkta rol oynayan dominant I genine, Akdağ çeşidinin NL-4 hariç BCMV ve BCMNV'nin bilinen bütün ırklarına karşı dayanıklılığı sağlayan resesif bc-22 genine, Özeren Şeker ve Özeren Barbunya çeşitlerinin ise BCMV'nin dört (NL-1, NL-7, US-2, NL-2) ve BCMNV'nin NL-8 ırkına karşı dayanıklılığı sağlayan bc-12 genine sahip olduğu tespit edilmiştir.

References

  • Ali M.A. 1950. Genetics of resistance to the common bean mosaic virus in the bean (Phaseolus vulgaris L.). Phytopathology 40:69-79.
  • Clark M.R. and Adams A. M. 1977. Characteristics of the microplate method of EnzymeLinked Immunosorbent Assay for the detection of plant viruses. J.General Virology, 34:475-483.
  • Collmer C.W. Marston M.F. Taylor J.C. Jahn M.M. 2000. The I gene of bean: a dosagedependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus. Molecular. Plant-Microbe Interactions. 13:1266–1270.
  • Cooper L.I. and Jones A.T. 1983. Responses of plants to viruses: Proposals for the use of terms. Phytopathology 73, 127-128.
  • Coyne D.P. Steadman J.R. Godoy-Lutz G. Gilbertson R. Arnaud-Santana E. Beaver J.S. and Myers J.R. 2003. Contributions of the Bean/Cowpea CRSP to management of bean diseases. Field Crops Research, 82: 155-168.
  • Drijfhout E. 1978. Genetic interaction between Phaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. Doctoral thesis. Wageningen Agric. Res. Rep. 872.
  • Drijfhout, E. Silbernagel M.J. and Burke D.W. 1978. Differentiation of strains of Bean common mosaic virus. Neth. J. Plant Pathol., 84: 13-26.
  • Drijfhout E. 1994. Bean common mosaic virus (In: compendium of bean diseases, Second Edition. Ed: R. Hall). APS Press, St. Paul, Minnesota, USA.
  • Gilbertson R.L. Guzman P. Rojas M. Crnov R. and Mkandawıre A. 2001. Detection of bean-infecting viruses in California with an emphasis on the CRSP-facilitated work. Bean/Cowpea Collaborative Research Support Program-East Africa Proceedings: Bean Seed Workshop Arusha, Tanzania. pp. 4.
  • Haley S.D. Afanador L. and Kelly J.D. 1994a. Identificaton and application of random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathology 84, 157-160.
  • Haley S.D. Afanador L.K. and Kelly J.D. 1994b. Selection for monogenic resistance traits with coupling and repulsion phase RAPD markers. Crop Science 34, 1061-1066.
  • Johnson W.C. and Gepts P. 1994. Two molecular markers linked to bc-3. Bean Improvement Cooperative Annual Report 37, 206-207.
  • Johnson W.C. Guzman P. Mandala D. Mkandawıre A.B.C. Temple S. Gilbertson R.L. and Gepts P. 1997. Moleculer tagging of the bc-3 gene for introgression into Andean common bean. Crop Sci. 37: 248-254.
  • Kelly J.D. 1997. A review of varietal response to Bean common mosaic potyvirus in Phaseoulus vulgaris. Plant Var. Seeds, 10: 1-6.
  • Larsen R.C. Miklas P.N. Druffel K.L. and Wyatt S.D. 2005. NL3-K strain is a stable and naturally occuring interspesific recombinant derived from bean common mosaic necrosis virus and bean common mosaic virus. Phytopathology. Vol: 95. No. 9 1037-1042.
  • Melotto M. Afanador L. and Kelly J.D. 1996. Development of SCAR marker linked to the I gene in common bean. Genome 39, 1216-1219.
  • Miklas P.N. Afanador L. and Kelly J.D. 1996. Recombination facilitated RAPD marker assisted selection for disease resistance in common bean. Crop Science 36, 86-90.
  • Miklas P. N. Larsen R.C. Riley R. and Kelly J. D. 2000. Potential marker-assisted selection for bc-1² resistance to Bean common mosaic potyvirus in common bean. Euphytica, 116 (3): 211-219.
  • Mukeshimena G. Paneda A. Rodriguez-Suarez C. Ferreria J.J. Giraldez R. and Kelly J.D. 200 Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean. Euphytica. 144: 291-299. Myers J.R. Strausbaugh C.A. Forster R.L. and Mcclean P.E. 1996. Resistance and tolerance to bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in bean. Bean Improvement Cooperative Annual Report 39, 94-95.
  • Naderpour M. Lund O.S. Larsen R. Johansen E. 2010. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eIF4E allele. Molecualr Plant Pathology 11:255-263.
  • Ogliari J.B. and Castano M. 1992. Identification of Resistant Germplasm to the Bean common mosaic virus-BCMV. Pesquisa Agropecuaria Brasileira 27(7) :1043-1047. Sengooba T.N. Spence N.F. Walket D.G.A. Allen D.J. and Femi-Lana A. 1997. The occurrence of bean common mosaic necrosis virus in wild and forage legumes in Uganda. Plant Pathology. 46, 95-103.
  • Silbernagel M.J. 1995. Bean germplasm releases. Annu.Rep. Bean Improv. Coop. 38: 177.
There are 22 citations in total.

Details

Primary Language Tr
Journal Section Makaleler
Authors

İlyas Deligöz

Miray Arlı Sökmen This is me

Publication Date July 3, 2013
Submission Date July 3, 2013
Published in Issue Year 2013 Volume: 53 Issue: 2

Cite

APA Deligöz, İ., & Arlı Sökmen, M. (2013). Determination of resistance to Bean common mosaıc virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods. Plant Protection Bulletin, 53(2), 101-113.
AMA Deligöz İ, Arlı Sökmen M. Determination of resistance to Bean common mosaıc virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods. Plant Protection Bulletin. July 2013;53(2):101-113.
Chicago Deligöz, İlyas, and Miray Arlı Sökmen. “Determination of Resistance to Bean Common mosaıc virus (BCMV) and Bean Common Mosaic Necrosis virus (BCMNV) in Some Common Bean Genotypes Using Qualitative, Quantitative and Molecular Methods”. Plant Protection Bulletin 53, no. 2 (July 2013): 101-13.
EndNote Deligöz İ, Arlı Sökmen M (July 1, 2013) Determination of resistance to Bean common mosaıc virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods. Plant Protection Bulletin 53 2 101–113.
IEEE İ. Deligöz and M. Arlı Sökmen, “Determination of resistance to Bean common mosaıc virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods”, Plant Protection Bulletin, vol. 53, no. 2, pp. 101–113, 2013.
ISNAD Deligöz, İlyas - Arlı Sökmen, Miray. “Determination of Resistance to Bean Common mosaıc virus (BCMV) and Bean Common Mosaic Necrosis virus (BCMNV) in Some Common Bean Genotypes Using Qualitative, Quantitative and Molecular Methods”. Plant Protection Bulletin 53/2 (July 2013), 101-113.
JAMA Deligöz İ, Arlı Sökmen M. Determination of resistance to Bean common mosaıc virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods. Plant Protection Bulletin. 2013;53:101–113.
MLA Deligöz, İlyas and Miray Arlı Sökmen. “Determination of Resistance to Bean Common mosaıc virus (BCMV) and Bean Common Mosaic Necrosis virus (BCMNV) in Some Common Bean Genotypes Using Qualitative, Quantitative and Molecular Methods”. Plant Protection Bulletin, vol. 53, no. 2, 2013, pp. 101-13.
Vancouver Deligöz İ, Arlı Sökmen M. Determination of resistance to Bean common mosaıc virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) in some common bean genotypes using qualitative, quantitative and molecular methods. Plant Protection Bulletin. 2013;53(2):101-13.

136481365013649