Research Article
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Comparative pollen morphology studies on some species of Brassicaceae in Turkey

Year 2021, Volume: 14 Issue: 1, 105 - 118, 15.04.2021
https://doi.org/10.46309/biodicon.2021.773419

Abstract

Pollen aperture type, shape, size, polar axis length, equatorial axis length, P/E ratio, colpus length, thickness of the sexine and nexine, S/N ratio, ornamentation type, and lumen shape and width were determined by using light and scanning electron microscope. A multivariate analysis was carried out using the Gover’s general similarity coefficient and UPGMA based on 3 qualitative and 8 quantitative pollen characters for 43 taxa belonging to 30 genera and 19 tribes. In all of the taxa studied, the pollen grains were generally small or medium-sized, tricolpate, except in Matthiola longipetala, with an inconspicuous aperture. The shape varied from prolate-spheroidal to perprolate. The exine ornamentation was microreticulate, reticulate, or macroreticulate. In some species, reticulate ornamentation was found together with microreticulate, and in some others, with macroreticulate ornamentation. It was seen that some taxa belonging to some genera placed near each other in the dendogram partially supported some tribes, such as Brassiceae, Alysseae, Sisymbrieae, and Camelineae. It is notable that some closely-related tribes were placed near each other on the dendrogram, which partially supports some previous molecular studies. In addition to exine ornamentation, the pollen size, shape, sexine and nexine thickness, and colpus length were the most useful characters. These characteristics can be used to identify and distinguish some genera, species and partly tribes within the family. They also provide detailed information on the pollen grains of some Brassicaceae taxa, some of which were studied herein for the first time, and this knowledge will be useful for comparisons in future pollen studies.

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References

  • [1] Mummenhoff, K., & Zunk, K. (1991). Should Thlaspi (Brassicaceae) be split? Preliminary evidence from isoelectric focusing analysis of Rubisco. Taxon, 40, 427- 434.
  • [2] Mummenhoff, K., & Koch, M. (1994). Chloroplast DNA restriction site variation and phylogenetic relationships in the genus Thlaspi sensu lato Brassicaceae. Systematic Botany, 19, 73–88.
  • [3] Zunk, K., Mummenhoff, K., Koch, M., & Hurka, H. (1996). Phylogenetic relationships of Thlaspi s.l. (subtribe Thlaspidinae, Lepidieae) and allied genera based on chloroplast DNA restriction-site variation. Theoretical and Applied Genetics, 92, 375–381.
  • [4] Koch, M., & Al-Shehbaz, I.A. (2004). Taxonomic and phylogenetic evaluation of the American Thlaspi Species: identity and relationship to the Eurasian genus Noccaea (Brassicaceae). Systematic Botany, 29, 375–384.
  • [5] Carlo, D, & Paula, R. (2004). New insights into pollen evolution. International Journal of Plant Sciences, 164:835.
  • [6] Davis, P.H., & Heywood V.H. (1973). Principles of angiosperm taxonomy. Huntington, New York: Robert E. Kieger Publishing Co.
  • [7] Orcan, N., & Binzet, R. (2004). A study of Alyssum floribundum (Brassicaceae). Phytologia Balcanica, 10 (2-3), 217–225.
  • [8] Khalik, K.A., Van Den Berg, R.G., Van Der Maesen, L.J.G., & El Hadidi, M.N. (2002). Pollen morphology of some tribes of Brassicaceae from Egypt and its systematic implications. Feddes Repertorium, 113, 211–223.
  • [9] Erdtman, G. (1972). Pollen morphology and plant taxonomy. New York.
  • [10] Sharma, R.R. & Nair, P.K.K. (1973). A study on pollen production in Brassica L. Palynological Bull. V.J., 2, 62-66.
  • [11] Carter, A.L., Williams, S.T., & McNeilly, T. (1975). Scanning electron microscope studies of pollen behaviour on immature mature Brussels sprout (Brassica oleracea var. gemmifera) stigmas. Euphytica, 24, 133-141.
  • [12] Appel, O. and Al-Shehbaz, I.A. (2002). Cruciferae. In: The Families and Genera of Vascular Plants. Flowering Plant Dicotyledons, Malvales, Capparales and Non- Betalain Caryophyllaceae. Kubitzki, K and C. Bayer (Eds.) vol.5, 75-174. New York: Springer-Verlarg, Berlin Heidelberg, [13] Mutlu, B., & Erik, S. (2012). Pollen morphology and its taxonomic significance of the genus Arabis (Brassicaceae) in Turkey. Plant Systematics and Evolution, 298, 1931– 1946.
  • [14] Erdtman, G. (1952). Pollen morphology and plant taxonomy. Angiosperms. Stockholm: Almquist and Wiksell.
  • [15] Khan, R. (2004). Studies on the pollen morphology of the Genus Arabidopsis (Brassicaceae) from Pakistan. Pakistan Journal of Botany, 36(2), 229–234.
  • [16] Perveen, A., Qaiser, M., & Khan, R. (2004). Pollen flora of Pakistan-XLVII. Brassicaceae. Pakistan Journal of Botany, 36, 683–700.
  • [17] Sagun, V., & Auer, C. (2016). Pollen morphology of selected species in the Camelineae (Brassicaceae). Palynology, 41(2), 255–266.
  • [18] Ančev, M., & Deneva, B. (1997). Pollen morphology of seventeen species from family Brassicaceae (Cruciferae). Phytologia Balcanica, 3, 75-82.
  • [19] Doğan, C., & İnceoğlu, O. (1990). Pollen morphology of some Isatis L. taxa in Turkey. Turkish Journal of Botany, 14, 12-31.
  • [20] Brochmann, C. (1992). Pollen and seed anatomy of Nordic Draba (Brassicaceae phytogenetic and ecological implications. Nordic Journal of BotanyT, 12(6), 657-673.
  • [21] Pınar, N.M., Duran, A., Çeter, T., & Tuğ, G.N. (2009). Pollen and seed morphology of the genus Hesperis L. (Brassicaceae) in Turkey. Turkish Journal of Botany, 33, 83-96.
  • [22] Kızılpınar, İ., Altınözlü, H., & Doğan, C. (2012). Pollen morphology of the some species of the genus Malcolmia (Brassicaceae). Mellifera, 12(23), 24-29.
  • [23] Karaismailoğlu, M.C. (2017). Palynological features of eleven Aethionema taxa from Turkey and their systematic implications. Bangladesh Journal of Plant Taxonomy, 24(2), 197-204.
  • [24] Erdtman, G. (1954). An introduction to pollen analysis. Stockholm: Almqvist and Wiksell.
  • [25] Erdtman, G. (1960). The acetolysis method. A revised description. Svensk Botanisk Tidskrift. 54, 561–564.
  • [26] Menemen, Y., & Jury, S. L. (2001). A taxonomic revision of the genus Pastinaca L., (Umbelliferae). Israel Journal of Plant Sciences, 49: 67-77.
  • [27] Punt, W., Hoen, PP., Blackmore, S., Nilssony, S., & Le Thomas, A. (2007). Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology, 143, 1-81.
  • [28] Erdtman, G. (1943). An introduction to pollen analysis. Waltham, Mass: Chronica Botanica Company.
  • [29] Lahham, J. N., &Al-Eisawi, D. (1987). Pollen morphology of Jordanian Cruciferae. Mitteilungen der Botanischen Staatssammlung München. 23, 355–375.
  • [30] Rollins, R.C., & Banerjee, U.C. (1976). Trichomes in studies of the Cruciferae. In: Vaughan, J. G., MacLeod, A.J., Jones, B.M.J. (edlr.). The biology and chemistry of the Cruciferae. Academic Press, London.
  • [31] Jonsell, B. (1979). New taxa of Cruciferae from East tropical Africa and Madagascar. Botanisca Notiser, 132, 521–544.
  • [32] İnceoğlu, Ö., & Karamustafa, F. (1975). The pollen morphology of plants in Ankara region II. Cruciferae. Commommunication Faculty of Sciences University of Ankara Series, C2, 21, 111–118.
  • [33] Kiefer, M., Schmickl, R., German, D.A., Mandáková, T., Lysak, M.A., Al-Shehbaz, I.A., Franzke, A., Mummenhoff, K., Stamatakis, A., & Koch, M.A. (2014). BrassiBase: Introduction to a novel knowledge database on Brassicaceae evolution. Plant Cell Physiology, 55(1), 1‒9.
  • [34] Çıtak, B.Y., Dural, H., & Gönen., B. (2016). Selçuk Üniversitesi Alâeddin Keykubat Kampüsü’nde Yayılış Gösteren Bazı Bitkilerin Polen Morfolojileri. Fen Fakültesi Fen Dergisi, 42 (1), 42-56.
  • [35] Çetin, Ö., Duran, A., Martin, E., & Tuştaş, S. (2012). A taxonomic study of the genus Fibigia Medik. (Brassicaceae). African Journal of Biotechnology, 11(1), 109-119.

Türkiye’deki bazı Brassicaceae türleri üzerine karşılaştırmalı polen morfolojisi çalışmaları

Year 2021, Volume: 14 Issue: 1, 105 - 118, 15.04.2021
https://doi.org/10.46309/biodicon.2021.773419

Abstract

Polen apertür tipi, şekli, boyutu, polar eksen uzunluğu, ekvatoral eksen uzunluğu, P/E oranı, kolpus uzunluğu, sekzin ve nekzin kalınlığı, S/N oranı, süslenme tipi, lümen şekli ve genişliği ışık ve taramalı electron mikroskobu kullanılarak belirlenmiştir. 19 oymağa ait 30 cinsten 43 takson için 3 nitel ve 8 nicel polen karakterine dayalı olarak Gover’s genel benzerlik katsayısı ve UPGMA kullanılarak çok değişkenli bir analiz gerçekleştirilmiştir. İncelenen tüm taksonlarda, polen taneleri genellikle küçük veya orta büyüklükte ve trikolpat olup, Matthiola longipetala’nın dışında belirgin bir apertüre sahiptir. Polen şekli prolat-sferoidalden perprolata kadar değişiklik göstermiştir. Ekzin süslenmesi mikroretikülat, retikülat veya makroretikülattır. Bazı türlerde retikülat süslenme mikroretikülat ile, bazı türlerde de makroretikülat süslenme ile birlikte bulunmuştur. Bazı cinslere ait taksonların dendogram üzerinde birbirine yakın olarak konumlanışı, Brassiceae, Alysseae, Sisymbrieae ve Camelineae gibi bazı oymakların kısmen desteklendiğini göstermektedir. Dendrogramda birbirine yakın konumlanan yakın akraba olan bazı oymakların bazı önceki moleküler çalışmalarda kısmen desteklenmesi dikkate değerdir. Ekzin süslenmesine ek olarak, polen boyutu, şekli, sekzin ve nekzin kalınlığı ve kolpus uzunluğu en kullanışlı karakterlerdir. Bu karakterler familya içindeki bazı cinsleri, türleri kısmen de oymakları tanımlamak ve ayırt etmek için kullanılabilir. Bu karakterler ayrıca, bazıları burada ilk kez incelenen bazı Brassicaceae taksonlarının polen taneleri hakkında ayrıntılı bilgi sağlamakta olup, elde edilen bu bilgiler gelecekteki polen çalışmalarında karşılaştırmalar için yararlı olacaktır.

Project Number

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References

  • [1] Mummenhoff, K., & Zunk, K. (1991). Should Thlaspi (Brassicaceae) be split? Preliminary evidence from isoelectric focusing analysis of Rubisco. Taxon, 40, 427- 434.
  • [2] Mummenhoff, K., & Koch, M. (1994). Chloroplast DNA restriction site variation and phylogenetic relationships in the genus Thlaspi sensu lato Brassicaceae. Systematic Botany, 19, 73–88.
  • [3] Zunk, K., Mummenhoff, K., Koch, M., & Hurka, H. (1996). Phylogenetic relationships of Thlaspi s.l. (subtribe Thlaspidinae, Lepidieae) and allied genera based on chloroplast DNA restriction-site variation. Theoretical and Applied Genetics, 92, 375–381.
  • [4] Koch, M., & Al-Shehbaz, I.A. (2004). Taxonomic and phylogenetic evaluation of the American Thlaspi Species: identity and relationship to the Eurasian genus Noccaea (Brassicaceae). Systematic Botany, 29, 375–384.
  • [5] Carlo, D, & Paula, R. (2004). New insights into pollen evolution. International Journal of Plant Sciences, 164:835.
  • [6] Davis, P.H., & Heywood V.H. (1973). Principles of angiosperm taxonomy. Huntington, New York: Robert E. Kieger Publishing Co.
  • [7] Orcan, N., & Binzet, R. (2004). A study of Alyssum floribundum (Brassicaceae). Phytologia Balcanica, 10 (2-3), 217–225.
  • [8] Khalik, K.A., Van Den Berg, R.G., Van Der Maesen, L.J.G., & El Hadidi, M.N. (2002). Pollen morphology of some tribes of Brassicaceae from Egypt and its systematic implications. Feddes Repertorium, 113, 211–223.
  • [9] Erdtman, G. (1972). Pollen morphology and plant taxonomy. New York.
  • [10] Sharma, R.R. & Nair, P.K.K. (1973). A study on pollen production in Brassica L. Palynological Bull. V.J., 2, 62-66.
  • [11] Carter, A.L., Williams, S.T., & McNeilly, T. (1975). Scanning electron microscope studies of pollen behaviour on immature mature Brussels sprout (Brassica oleracea var. gemmifera) stigmas. Euphytica, 24, 133-141.
  • [12] Appel, O. and Al-Shehbaz, I.A. (2002). Cruciferae. In: The Families and Genera of Vascular Plants. Flowering Plant Dicotyledons, Malvales, Capparales and Non- Betalain Caryophyllaceae. Kubitzki, K and C. Bayer (Eds.) vol.5, 75-174. New York: Springer-Verlarg, Berlin Heidelberg, [13] Mutlu, B., & Erik, S. (2012). Pollen morphology and its taxonomic significance of the genus Arabis (Brassicaceae) in Turkey. Plant Systematics and Evolution, 298, 1931– 1946.
  • [14] Erdtman, G. (1952). Pollen morphology and plant taxonomy. Angiosperms. Stockholm: Almquist and Wiksell.
  • [15] Khan, R. (2004). Studies on the pollen morphology of the Genus Arabidopsis (Brassicaceae) from Pakistan. Pakistan Journal of Botany, 36(2), 229–234.
  • [16] Perveen, A., Qaiser, M., & Khan, R. (2004). Pollen flora of Pakistan-XLVII. Brassicaceae. Pakistan Journal of Botany, 36, 683–700.
  • [17] Sagun, V., & Auer, C. (2016). Pollen morphology of selected species in the Camelineae (Brassicaceae). Palynology, 41(2), 255–266.
  • [18] Ančev, M., & Deneva, B. (1997). Pollen morphology of seventeen species from family Brassicaceae (Cruciferae). Phytologia Balcanica, 3, 75-82.
  • [19] Doğan, C., & İnceoğlu, O. (1990). Pollen morphology of some Isatis L. taxa in Turkey. Turkish Journal of Botany, 14, 12-31.
  • [20] Brochmann, C. (1992). Pollen and seed anatomy of Nordic Draba (Brassicaceae phytogenetic and ecological implications. Nordic Journal of BotanyT, 12(6), 657-673.
  • [21] Pınar, N.M., Duran, A., Çeter, T., & Tuğ, G.N. (2009). Pollen and seed morphology of the genus Hesperis L. (Brassicaceae) in Turkey. Turkish Journal of Botany, 33, 83-96.
  • [22] Kızılpınar, İ., Altınözlü, H., & Doğan, C. (2012). Pollen morphology of the some species of the genus Malcolmia (Brassicaceae). Mellifera, 12(23), 24-29.
  • [23] Karaismailoğlu, M.C. (2017). Palynological features of eleven Aethionema taxa from Turkey and their systematic implications. Bangladesh Journal of Plant Taxonomy, 24(2), 197-204.
  • [24] Erdtman, G. (1954). An introduction to pollen analysis. Stockholm: Almqvist and Wiksell.
  • [25] Erdtman, G. (1960). The acetolysis method. A revised description. Svensk Botanisk Tidskrift. 54, 561–564.
  • [26] Menemen, Y., & Jury, S. L. (2001). A taxonomic revision of the genus Pastinaca L., (Umbelliferae). Israel Journal of Plant Sciences, 49: 67-77.
  • [27] Punt, W., Hoen, PP., Blackmore, S., Nilssony, S., & Le Thomas, A. (2007). Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology, 143, 1-81.
  • [28] Erdtman, G. (1943). An introduction to pollen analysis. Waltham, Mass: Chronica Botanica Company.
  • [29] Lahham, J. N., &Al-Eisawi, D. (1987). Pollen morphology of Jordanian Cruciferae. Mitteilungen der Botanischen Staatssammlung München. 23, 355–375.
  • [30] Rollins, R.C., & Banerjee, U.C. (1976). Trichomes in studies of the Cruciferae. In: Vaughan, J. G., MacLeod, A.J., Jones, B.M.J. (edlr.). The biology and chemistry of the Cruciferae. Academic Press, London.
  • [31] Jonsell, B. (1979). New taxa of Cruciferae from East tropical Africa and Madagascar. Botanisca Notiser, 132, 521–544.
  • [32] İnceoğlu, Ö., & Karamustafa, F. (1975). The pollen morphology of plants in Ankara region II. Cruciferae. Commommunication Faculty of Sciences University of Ankara Series, C2, 21, 111–118.
  • [33] Kiefer, M., Schmickl, R., German, D.A., Mandáková, T., Lysak, M.A., Al-Shehbaz, I.A., Franzke, A., Mummenhoff, K., Stamatakis, A., & Koch, M.A. (2014). BrassiBase: Introduction to a novel knowledge database on Brassicaceae evolution. Plant Cell Physiology, 55(1), 1‒9.
  • [34] Çıtak, B.Y., Dural, H., & Gönen., B. (2016). Selçuk Üniversitesi Alâeddin Keykubat Kampüsü’nde Yayılış Gösteren Bazı Bitkilerin Polen Morfolojileri. Fen Fakültesi Fen Dergisi, 42 (1), 42-56.
  • [35] Çetin, Ö., Duran, A., Martin, E., & Tuştaş, S. (2012). A taxonomic study of the genus Fibigia Medik. (Brassicaceae). African Journal of Biotechnology, 11(1), 109-119.
There are 34 citations in total.

Details

Primary Language English
Subjects Conservation and Biodiversity
Journal Section Research Article
Authors

Ayşe Erden 0000-0002-8990-0616

Yusuf Menemen 0000-0002-9546-7986

Project Number -
Publication Date April 15, 2021
Submission Date July 26, 2020
Acceptance Date March 19, 2021
Published in Issue Year 2021 Volume: 14 Issue: 1

Cite

APA Erden, A., & Menemen, Y. (2021). Comparative pollen morphology studies on some species of Brassicaceae in Turkey. Biological Diversity and Conservation, 14(1), 105-118. https://doi.org/10.46309/biodicon.2021.773419

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