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MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY

Year 2012, Volume: 2 Issue: 1, 31 - 39, 21.03.2012

Nalan Yılmaz Sarıözlü Rasime Demirel Merih Kıvanç

Abstract

In order to investigate and find out the bacterial community of hot spring waters in Eskişehir,

Turkey, 7 hot spring water samples were collected from 7 different hot springs. All samples were inoculated using four different media (nutrient agar, water yeast extract agar, trypticase soy agar, starch casein agar). After incubation at 50 ºC for 14 days, all bacterial colonies were counted and purified. Gram reaction, catalase and oxidase properties of all isolates were determined and investigated by BIOLOG, VITEK and automated ribotyping system (RiboPrinter). The resistance of these bacteria was examined against ampiciline, gentamisine, trimethoprime-sulphamethoxazole and tetracycline. As a result, heat resistant pathogenic microorganisms in addition to human normal flora were determined in hot spring waters (43-50 ºC) in investigated area. Ten different species belong to 6 genera were identified as Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans and Pseudomonas taetrolens.

Keywords

Hot spring; Eskişehir; BIOLOG; VITEK; Ribotype.

References

  • Anonymous (2009). Thermal Springs. (http://turkeytravelresource.com/view/253 /thermal-springs--).
  • Baker, G.C., Gaffar, S., Cowan, D.A. and Suharto, A.R. (2001). Bacterial commu- nity analysis of Indonesian hot springs. FEMS Microbiology Letters 200, 103- 109.
  • Belkova, N.L., Tazaki, K., Zakharova, J.R. and Parfenova, V.V. (2007). Activity of bacte- ria in water of hot springs from Southern and Central Kamchatskaya geothermal provinces, Kamchatka Peninsula, Russia. Microbiological Research. 162, 99-107.
  • Bsop I.D.11 (2007). Identification of Moraxella Species and Morphologically Similar Or- ganisms. Issue No: 2, Issue Date: 14.08.07, Issue by: Standards Unit, Evalu- tions and Standards Laboratory 1-13.
  • Boone, D.R., Castenholz, R.W., Bergey, D.H., Garrity, G.M. and Staley, J.T. (2001). Bergey's Manual of Systematic Bacteriol- ogy, Second Edition, Springer, New York.
  • Cordevant, C., Tang, J.S., Cleland, D. and Lange, M. (2003). Characterization of members of the Legionellaceae family by automated ribotyping. Journal of Clinical Microbiology 41(1), 34-43.
  • El-Banna, N.M. (2007). Antifungal activity of Comamonas acidovorans isolated from water pond in south Jordan. African Jour- nal of Biotechnology 6(19), 2216-2219.
  • Enright, M.C., Carter, P.E., MacLean, I.A. and McKenzie, H. (1994). Phylogenetic rela- tionships between some members of the genera Moraxella and Kingella based on partial 16S ribosomal DNA sequence analysis. International Journal of Systematic Bacteriology 44(3), 387-391.
  • Helsel, L.O., Hollis, D.G., Steigerwalt, A.G. and Levett, P.N. (2006). Reclassification of Roseomonas fauriae Rihs et al.1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. Interna- tional Journal of Systematic and Evolu- tionary Microbiology 56, 2753-2755.
  • Hiraishi, A., Umezawa, T. Yamamoto, H., Kato, K. and Maki, Y. (1999). Changes in qui- none profiles of hot spring microbial mats with a thermal gradient. Applied and En- vironmental Microbiology 65(1), 198- 205.
  • Ito, Y., Linuma, Y., Baba, H., Sugino, Y., Hasegawa, Y., Shimokata, K., Ichiyama, S., Hasegawa, T. and Ohta, M. (2003). Evaluation of automated ribotyping sys- tem for characterization and identification of verocytotoxin-producing Escherichia coli isolated in Japan. Japanese Journal of Infectious Disease 56(5-6), 200-204.
  • Laboratory Manager Vitek Manual (2004). TML/MSH Microbiology Department Policy (http://microbiology.mstinai.on.ca/manual /vitek/mi_vitek.pdf). Manual.
  • Madigan, M.T. and Martinko, J.M. (2006). Brock Biology of Microorganisms, Elev- enth Edition, Pearson Prentice Hall.
  • OOA 002 (2001). Gram positive identification test panel (http://www.biolog.com/).
  • OOA 003 (2001). Gram negative identification test panel (http://www.biolog.com/).
  • Stepanović, S., Vuković, D., Bedora-Faure, M., K’ouas, G., Djukić, S., Švabić-Vlahović, M. and Carlier, J.P. (2007). Surgical wound Psychrobacter phenylpyruvicus-like or- ganism. Diagnostic Microbiology and Infectious Disease 57, 217-219. with
  • Sternberg, M. and Lockwood, L.B. (1969). Oxi- dation of isomaltose by Pseudomonas tae- trolens. Journal of Bacteriology 99(2), 623.
  • Tompkin, R.B. and Shaparis, A.B. (1972). Po- tato aroma of lamb carcasses. Applied Mi- crobiology. 24(6), 1003-1004.
  • Wahlund, T.M., Woese, C.R., Castenholz, R.W. and Madigan, M.T. (1991). A thermo- philic green sulfur bacterium from New Zealand hot springs, Chlorobium tepidum sp. nov., Arch Microbiol. 156, 81-90.
  • Wistreich, G.A. (1997). Microbiology Labora- tory Fundamental and applications, Pren- tice Hall, US. 5

Yaşam Bilimleri ve Biyoteknoloji

Year 2012, Volume: 2 Issue: 1, 31 - 39, 21.03.2012

Nalan Yılmaz Sarıözlü Rasime Demirel Merih Kıvanç

Abstract

Eskişehir/Türkiye’deki ılıca sularındaki bakteriyel topluluğun ortaya konulması ve incelenmesi için 7 ılıca su örneği 7 farklı ılıcadan toplanmıştır. Tüm örnekler dört farklı besi ortamına (nutrient agar, su-maya ekstraktı agar, triptik soy agar, nişasta-kazein agar) inoküle edilmiştir. 50 °C’de 14 günlük inkübasyondan sonra tüm bakteri kolonilerinin sayımı yapılıp saflaştırılmıştır. Tüm izolatların gram reaksiyonları, katalaz veoksidazözellikleri belirlenmişve BIOLOG, VITEK veotomatikribotiplendirme sisteminde (RiboPrinter) incelenmişlerdir. Bu bakterilerin ampisilin, gentamisin, trimetoprim-sülfametoksazol ve tetrasiklin antibiyotiklerine karşı dirençliliği incelenmiştir. Sonuç olarak normal insan florasına ilaveten ısıya dirençli patojenik mikroorganizmalar incelenen alandaki ılıca sularında (43-50 °C) belirlenmiştir. Altı genusa ait 10 farklı tür Alysiella filiformis, Bordetella bronchiseptica, B. pertussis, Molexalla caprae, M. caviae, M. cuniculi, M. phenylpyruvica, Roseomonas fauriae, Delftia acidovorans ve Pseudomonas taetrolens olarak tanımlanmıştır

Keywords

Ilıca Eskişehir BIOLOG VITEK Ribotip

References

  • Anonymous (2009). Thermal Springs. (http://turkeytravelresource.com/view/253 /thermal-springs--).
  • Baker, G.C., Gaffar, S., Cowan, D.A. and Suharto, A.R. (2001). Bacterial commu- nity analysis of Indonesian hot springs. FEMS Microbiology Letters 200, 103- 109.
  • Belkova, N.L., Tazaki, K., Zakharova, J.R. and Parfenova, V.V. (2007). Activity of bacte- ria in water of hot springs from Southern and Central Kamchatskaya geothermal provinces, Kamchatka Peninsula, Russia. Microbiological Research. 162, 99-107.
  • Bsop I.D.11 (2007). Identification of Moraxella Species and Morphologically Similar Or- ganisms. Issue No: 2, Issue Date: 14.08.07, Issue by: Standards Unit, Evalu- tions and Standards Laboratory 1-13.
  • Boone, D.R., Castenholz, R.W., Bergey, D.H., Garrity, G.M. and Staley, J.T. (2001). Bergey's Manual of Systematic Bacteriol- ogy, Second Edition, Springer, New York.
  • Cordevant, C., Tang, J.S., Cleland, D. and Lange, M. (2003). Characterization of members of the Legionellaceae family by automated ribotyping. Journal of Clinical Microbiology 41(1), 34-43.
  • El-Banna, N.M. (2007). Antifungal activity of Comamonas acidovorans isolated from water pond in south Jordan. African Jour- nal of Biotechnology 6(19), 2216-2219.
  • Enright, M.C., Carter, P.E., MacLean, I.A. and McKenzie, H. (1994). Phylogenetic rela- tionships between some members of the genera Moraxella and Kingella based on partial 16S ribosomal DNA sequence analysis. International Journal of Systematic Bacteriology 44(3), 387-391.
  • Helsel, L.O., Hollis, D.G., Steigerwalt, A.G. and Levett, P.N. (2006). Reclassification of Roseomonas fauriae Rihs et al.1998 as a later heterotypic synonym of Azospirillum brasilense Tarrand et al. 1979. Interna- tional Journal of Systematic and Evolu- tionary Microbiology 56, 2753-2755.
  • Hiraishi, A., Umezawa, T. Yamamoto, H., Kato, K. and Maki, Y. (1999). Changes in qui- none profiles of hot spring microbial mats with a thermal gradient. Applied and En- vironmental Microbiology 65(1), 198- 205.
  • Ito, Y., Linuma, Y., Baba, H., Sugino, Y., Hasegawa, Y., Shimokata, K., Ichiyama, S., Hasegawa, T. and Ohta, M. (2003). Evaluation of automated ribotyping sys- tem for characterization and identification of verocytotoxin-producing Escherichia coli isolated in Japan. Japanese Journal of Infectious Disease 56(5-6), 200-204.
  • Laboratory Manager Vitek Manual (2004). TML/MSH Microbiology Department Policy (http://microbiology.mstinai.on.ca/manual /vitek/mi_vitek.pdf). Manual.
  • Madigan, M.T. and Martinko, J.M. (2006). Brock Biology of Microorganisms, Elev- enth Edition, Pearson Prentice Hall.
  • OOA 002 (2001). Gram positive identification test panel (http://www.biolog.com/).
  • OOA 003 (2001). Gram negative identification test panel (http://www.biolog.com/).
  • Stepanović, S., Vuković, D., Bedora-Faure, M., K’ouas, G., Djukić, S., Švabić-Vlahović, M. and Carlier, J.P. (2007). Surgical wound Psychrobacter phenylpyruvicus-like or- ganism. Diagnostic Microbiology and Infectious Disease 57, 217-219. with
  • Sternberg, M. and Lockwood, L.B. (1969). Oxi- dation of isomaltose by Pseudomonas tae- trolens. Journal of Bacteriology 99(2), 623.
  • Tompkin, R.B. and Shaparis, A.B. (1972). Po- tato aroma of lamb carcasses. Applied Mi- crobiology. 24(6), 1003-1004.
  • Wahlund, T.M., Woese, C.R., Castenholz, R.W. and Madigan, M.T. (1991). A thermo- philic green sulfur bacterium from New Zealand hot springs, Chlorobium tepidum sp. nov., Arch Microbiol. 156, 81-90.
  • Wistreich, G.A. (1997). Microbiology Labora- tory Fundamental and applications, Pren- tice Hall, US. 5
There are 20 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Nalan Yılmaz Sarıözlü

Rasime Demirel This is me

Merih Kıvanç

Publication Date March 21, 2012
Published in Issue Year 2012 Volume: 2 Issue: 1

Cite

APA Yılmaz Sarıözlü, N., Demirel, R., & Kıvanç, M. (2012). MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 2(1), 31-39.
AMA Yılmaz Sarıözlü N, Demirel R, Kıvanç M. MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. March 2012;2(1):31-39.
Chicago Yılmaz Sarıözlü, Nalan, Rasime Demirel, and Merih Kıvanç. “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 2, no. 1 (March 2012): 31-39.
EndNote Yılmaz Sarıözlü N, Demirel R, Kıvanç M (March 1, 2012) MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 2 1 31–39.
IEEE N. Yılmaz Sarıözlü, R. Demirel, and M. Kıvanç, “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, vol. 2, no. 1, pp. 31–39, 2012.
ISNAD Yılmaz Sarıözlü, Nalan et al. “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 2/1 (March 2012), 31-39.
JAMA Yılmaz Sarıözlü N, Demirel R, Kıvanç M. MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2012;2:31–39.
MLA Yılmaz Sarıözlü, Nalan et al. “MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, vol. 2, no. 1, 2012, pp. 31-39.
Vancouver Yılmaz Sarıözlü N, Demirel R, Kıvanç M. MICROBIAL POPULATION OF HOT SPRING WATERS IN ESKİŞEHİR/TURKEY. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2012;2(1):31-9.
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