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DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA

Year 2011, Volume: 26 Issue: 2, 13 - 26, 20.02.2013

Abstract

Natural bacterial biota of European flat oyster, 60 individual Ostrea edulis samples collected from the northern coast of the Marmara Sea, for each month from November 2009 to April 2010 were investigated in this study. Sixty oyster were harvested along 500 m of shallow (10-15 m depth) using a mechanical oyster dredge from natural growing areas. Totally 44 bacterial strains were isolated and identified by using conventional bacteriologic methods and API 20E commercial rapid identification kit. On the basis of the above mentioned tests, the isolates were identified as a member of the genera Vibrio, Shewanella and Citrobacter. Shewanella putrefaciens was mostly isolated species. The other isolated bacterial species were Vibrio fluvialis, V. vulnificus, V. alginolyticus and Citrobacter freundii in this study. S. putrefaciens could pose potential health risk and could be transmitted to humans via food consumption or swimming in contaminated water. For these reasons, its isolation from oysters samples is important for human health.

References

  • Altuğ G, Çardak M, Çiftçi PS (2008). Indicator and other bacteria in striped venus (Chamelea gallina, L.) and wedge clam (Donax trunculus) from the northern coast of the sea of Marmara, Turkey. Journal of ShellŞsh Research, 27 (4), 783-788.
  • Ateş C, (1998). Development of Oyster (Ostrea edulis L. 1758) in the Dardanelles and in the Northern part of Marmara. PhD thesis, İstanbul Üniversity, Science Enstituation, 88 pp (in Turkish with English abstract).
  • Bowman JP (2005). Shewanella in Bergey’s Manual of Systematic Bacteriology. BRENNER DJ., KRIEG NR., STALEY J T., Volume 2, 480- 491, Springer.
  • Buck ID, Wells RS, Rhinehart HL, Hansen LJ (2006). Aerobic micro- organisms associated with Free-Ranging Bottlenose Dolphins in Coastal Gulf of Mexico and Atlantic Ocean Waters. J Wildl Dis, 42 (3), 536-544.
  • Campbell MS, Wright AC (2003). Real Time PCR Analysis of Vibrio vulniŞcus from Oysters. Appl Environ Microbiol, 69 (12), 7137-7144.
  • Chakraborty R, Sinha S, Mukhopadhyay AK, Sakura MA, Yamasaki S, Bhattacharya SK, Balakrish Nair G, Ramamurthy T (2006). Species- speciŞc identiŞcation of Vibrioşuvialis by PCR targeted to the conserved transcriptional activation and variable membrane tether regions of the tox R gene. J Med Microbiol, 55, 805-808.
  • Daniels NA, Ray B, Elston A (2000). Emergence of a new Vibrio para- haemolyticus Serotype in raw oysters: A prevention quandary. JAMA, 284 (12),1541-1545.
  • Farmer JJ, J anda JM (2005). Vibrionaceae in Bergey’s Manual of Syste- matic Bacteriology. BRENNER DJ ., KRIEG NR., STALEY J T., Volume 2, 491-546, Springer.
  • Frederiksen W (2005). Citrobacter in Bergey’s Manual of Systematic Bacteriology. Brenner, D.J ., Krieg, N.R., Staley, J .T., Volume 2, 651-656, Springer.
  • Grizzle JM, Brunner CJ (2009). Infectious diseases of freshwater mus- sels and other freshwater bivalve mollusks. Rev Fish Sci, 17 (4), 425-467.
  • J aziri H (1990). Variations ge'nétiques et structuration biogéographique chez un bivalve marin: I’huître plate Ostrea edulis L.(PhD dissertation) Montpellier, France: University of Montpellier.
  • Jones DD, Law R, Bej AK (1993). Detection of Salmonella spp. in Oys- ters using polymerase chain reactions (PCR) and gene probes. Journal of Food Science, 58 (6), 1191-1197.
  • Korun J, Akgün-Dar K, Yazıcı M (2009). Isolation of Shewanella putre- faciens from cultured european sea bass, (Dicentrarchus labrax) in Turkey. Revue Med. Vet., 160 (11), 532-536.
  • La Valley KJ, Dealteris J, Rice M, Gomez-Chiarri M., (2008). North Atlantic Vibrio vulnijîcus surveillance from postharvest oysters at a US shellŞsh processing facility. Journal of Food Service, 19, 234-237.
  • Leblanc L, Leroi F, Hartke A, AuffrayY (2000). Do stresses encountered during the smoked salmon process inşuence the survival of the spoilng bacterium Shewanella putrefaciens?. Lett Appl Microbiol, 30, 437-442.
  • Macian KC, Ludwig W, Aznar R, Grimont PAD, Schleifer KH, Garay E, Pujalte MJ (2001). Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol, 51, 1449-1456.
  • Maugeri TL, Caccamo D, Gugliandolo C (2000). Potentially pathogenic Vibrios in brackish waters and mussels. J Appl Microbiol, 89, 261-266.
  • Montes J, Ferro-Soto B, Conchas RF, Guerra A (2003). Determining culture strategies in populations of the European flat oyster, Ostrea edulis, affected by bonamiosis. Science Direct, 220, 175-182.
  • Morris JG (2003). Cholera and other types of Vibriosis: A story of hu- man pandemics and oyster on the half shell. Clinical Infectious Diseases,
  • Najiah M, Nadirah M, Lee KI, Lee SW, Wendy W, Ruhil HH, Nurul FA (2008). Bacteria şora and heavy metals in cultivated oysters Crassostrea iraa’elei of Setiu Wetland, East Coast Peninsular Malaysia. Vet Res Com- mun, 32, 377-381.
  • Noguerola I, Blanch AR (2008). IdentiŞcation of Vibrio spp. with a set of dichotomous keys. J Appl Microbiol, 105, 175-185.
  • Papanaoum K, Marshman G, Gordon LA, Lumb R, Gordon DL (1998). Concurrent infection due to Shewanella putrefaciens and Mycobacterium marinum acquired at the beach. Aust J Dermatol, 39, 92-95.
  • Parvathi A, Kumar HS, KarunasagarI (2005). Study of the occurence of Vibrio vulniŞcus in oysters in India by polymerase chain reaction (PCR) and heterogeity among V. vulniŞcus by randomly ampliffŞed polymorphic DNA and gyrB sequence analysis. Environmental Microbiology, 7 (7), 995-1002.
  • Parveen S, Hettiarachchi KA, Bowers JC, Jones IL, Tamplin ML, McKay R, Beatty W, Brohawn K, Dasilva LV, Depaola A (2008). Seasonal distri- bution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters. Int J Food Microbiol, 128, 354-361.
  • Pujalte MJ , Ortigosa M, Macian MC, Garay E (1999). Aerobic and facultative anaerobic heterotrophic bacteria associated to Mediterranean oysters and seawater. Internatl Microbiol, 2, 259- 266.
  • Richards GP, Watson MA, Burt IG, Bushek D (2008). Shewanella and Photobacterium spp. in oysters and seawater from the Delaware Bay. Appl Environ Microbiol, 74 (11), 3323-3327.
  • Ripabelli G, Sammarco lVIL, McLauchlin J, Fanelli I (2003). Molecular Characterisation and Antimicrobial Resistance of Vibrio vulniŞcus and Vib- rio alginolyticus Isolated from Mussels (Mytilus galloprovincialis). System Appl Microbiol, 26, 1 19- 126.
  • Şeker E, Sarıeyyüpoğlu M (2003). IdentiŞcation of Salmonella Isolated from Freshwater Mussels (Unio elongatulus eucirrus Bourguignat, 1860) by Polymerase Chain Reaction. T urkJ VetAnim Sci, 27, 201-206.
  • Shehane SD, Sizemore RK (2002). Isolation and preliminary characte- rization of bacteriocins produced by Vibrio vulniŞcus. J Appl Microbial,
  • Sousa OV, Vieira RHSF, Menezes FGR, Reis CMF, Hofer E (2004). Detection of Vibrio parahaemolyticus and Vibrio cholerae in Oyster, Cras- sostrea rhizophorae, Collected from a Natural Nursery in the Cocö River Estuary, Cearâ, Brazil. Rev. Inst. Med. trop. S. Paulo, 46 (2), 59-62.
  • Uyan O, Aral O (2000). A Study on the possibilities of obtaining larva from Native Flat Oysters (Ostrea edulis L.) living in the Black Sea and larval metamorphosis stage. Turk] Zool, 24, 343- 350.
  • Vasconcelos GJ, Lee JS (1972). Microbial Flora of PaciŞc Oysters (Crassastrea gigas) Subjected to Ultraviolet-Irradiated Seawater. Appl Microbial, 23 (1), 11-16.
  • Venkateswaran K, Moser DP, Dollhopf ME, Lies DP, Saffarini DA, Macgregor BJ, Ringelberg DB, White DC, Nishij ima M, Sano H, Berghardt J, Stackebrandt E, Nealson, KH (1999). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol, 49, 705-724.
  • Williams HR, Macey BM, Burnett LE, Burnett KG (2008). Differential localization and bacteriostasis of Vibrio campbelli among tissues of the Eastern oyster, Crassastrea virginica, 1-9.
Year 2011, Volume: 26 Issue: 2, 13 - 26, 20.02.2013

Abstract

References

  • Altuğ G, Çardak M, Çiftçi PS (2008). Indicator and other bacteria in striped venus (Chamelea gallina, L.) and wedge clam (Donax trunculus) from the northern coast of the sea of Marmara, Turkey. Journal of ShellŞsh Research, 27 (4), 783-788.
  • Ateş C, (1998). Development of Oyster (Ostrea edulis L. 1758) in the Dardanelles and in the Northern part of Marmara. PhD thesis, İstanbul Üniversity, Science Enstituation, 88 pp (in Turkish with English abstract).
  • Bowman JP (2005). Shewanella in Bergey’s Manual of Systematic Bacteriology. BRENNER DJ., KRIEG NR., STALEY J T., Volume 2, 480- 491, Springer.
  • Buck ID, Wells RS, Rhinehart HL, Hansen LJ (2006). Aerobic micro- organisms associated with Free-Ranging Bottlenose Dolphins in Coastal Gulf of Mexico and Atlantic Ocean Waters. J Wildl Dis, 42 (3), 536-544.
  • Campbell MS, Wright AC (2003). Real Time PCR Analysis of Vibrio vulniŞcus from Oysters. Appl Environ Microbiol, 69 (12), 7137-7144.
  • Chakraborty R, Sinha S, Mukhopadhyay AK, Sakura MA, Yamasaki S, Bhattacharya SK, Balakrish Nair G, Ramamurthy T (2006). Species- speciŞc identiŞcation of Vibrioşuvialis by PCR targeted to the conserved transcriptional activation and variable membrane tether regions of the tox R gene. J Med Microbiol, 55, 805-808.
  • Daniels NA, Ray B, Elston A (2000). Emergence of a new Vibrio para- haemolyticus Serotype in raw oysters: A prevention quandary. JAMA, 284 (12),1541-1545.
  • Farmer JJ, J anda JM (2005). Vibrionaceae in Bergey’s Manual of Syste- matic Bacteriology. BRENNER DJ ., KRIEG NR., STALEY J T., Volume 2, 491-546, Springer.
  • Frederiksen W (2005). Citrobacter in Bergey’s Manual of Systematic Bacteriology. Brenner, D.J ., Krieg, N.R., Staley, J .T., Volume 2, 651-656, Springer.
  • Grizzle JM, Brunner CJ (2009). Infectious diseases of freshwater mus- sels and other freshwater bivalve mollusks. Rev Fish Sci, 17 (4), 425-467.
  • J aziri H (1990). Variations ge'nétiques et structuration biogéographique chez un bivalve marin: I’huître plate Ostrea edulis L.(PhD dissertation) Montpellier, France: University of Montpellier.
  • Jones DD, Law R, Bej AK (1993). Detection of Salmonella spp. in Oys- ters using polymerase chain reactions (PCR) and gene probes. Journal of Food Science, 58 (6), 1191-1197.
  • Korun J, Akgün-Dar K, Yazıcı M (2009). Isolation of Shewanella putre- faciens from cultured european sea bass, (Dicentrarchus labrax) in Turkey. Revue Med. Vet., 160 (11), 532-536.
  • La Valley KJ, Dealteris J, Rice M, Gomez-Chiarri M., (2008). North Atlantic Vibrio vulnijîcus surveillance from postharvest oysters at a US shellŞsh processing facility. Journal of Food Service, 19, 234-237.
  • Leblanc L, Leroi F, Hartke A, AuffrayY (2000). Do stresses encountered during the smoked salmon process inşuence the survival of the spoilng bacterium Shewanella putrefaciens?. Lett Appl Microbiol, 30, 437-442.
  • Macian KC, Ludwig W, Aznar R, Grimont PAD, Schleifer KH, Garay E, Pujalte MJ (2001). Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol, 51, 1449-1456.
  • Maugeri TL, Caccamo D, Gugliandolo C (2000). Potentially pathogenic Vibrios in brackish waters and mussels. J Appl Microbiol, 89, 261-266.
  • Montes J, Ferro-Soto B, Conchas RF, Guerra A (2003). Determining culture strategies in populations of the European flat oyster, Ostrea edulis, affected by bonamiosis. Science Direct, 220, 175-182.
  • Morris JG (2003). Cholera and other types of Vibriosis: A story of hu- man pandemics and oyster on the half shell. Clinical Infectious Diseases,
  • Najiah M, Nadirah M, Lee KI, Lee SW, Wendy W, Ruhil HH, Nurul FA (2008). Bacteria şora and heavy metals in cultivated oysters Crassostrea iraa’elei of Setiu Wetland, East Coast Peninsular Malaysia. Vet Res Com- mun, 32, 377-381.
  • Noguerola I, Blanch AR (2008). IdentiŞcation of Vibrio spp. with a set of dichotomous keys. J Appl Microbiol, 105, 175-185.
  • Papanaoum K, Marshman G, Gordon LA, Lumb R, Gordon DL (1998). Concurrent infection due to Shewanella putrefaciens and Mycobacterium marinum acquired at the beach. Aust J Dermatol, 39, 92-95.
  • Parvathi A, Kumar HS, KarunasagarI (2005). Study of the occurence of Vibrio vulniŞcus in oysters in India by polymerase chain reaction (PCR) and heterogeity among V. vulniŞcus by randomly ampliffŞed polymorphic DNA and gyrB sequence analysis. Environmental Microbiology, 7 (7), 995-1002.
  • Parveen S, Hettiarachchi KA, Bowers JC, Jones IL, Tamplin ML, McKay R, Beatty W, Brohawn K, Dasilva LV, Depaola A (2008). Seasonal distri- bution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters. Int J Food Microbiol, 128, 354-361.
  • Pujalte MJ , Ortigosa M, Macian MC, Garay E (1999). Aerobic and facultative anaerobic heterotrophic bacteria associated to Mediterranean oysters and seawater. Internatl Microbiol, 2, 259- 266.
  • Richards GP, Watson MA, Burt IG, Bushek D (2008). Shewanella and Photobacterium spp. in oysters and seawater from the Delaware Bay. Appl Environ Microbiol, 74 (11), 3323-3327.
  • Ripabelli G, Sammarco lVIL, McLauchlin J, Fanelli I (2003). Molecular Characterisation and Antimicrobial Resistance of Vibrio vulniŞcus and Vib- rio alginolyticus Isolated from Mussels (Mytilus galloprovincialis). System Appl Microbiol, 26, 1 19- 126.
  • Şeker E, Sarıeyyüpoğlu M (2003). IdentiŞcation of Salmonella Isolated from Freshwater Mussels (Unio elongatulus eucirrus Bourguignat, 1860) by Polymerase Chain Reaction. T urkJ VetAnim Sci, 27, 201-206.
  • Shehane SD, Sizemore RK (2002). Isolation and preliminary characte- rization of bacteriocins produced by Vibrio vulniŞcus. J Appl Microbial,
  • Sousa OV, Vieira RHSF, Menezes FGR, Reis CMF, Hofer E (2004). Detection of Vibrio parahaemolyticus and Vibrio cholerae in Oyster, Cras- sostrea rhizophorae, Collected from a Natural Nursery in the Cocö River Estuary, Cearâ, Brazil. Rev. Inst. Med. trop. S. Paulo, 46 (2), 59-62.
  • Uyan O, Aral O (2000). A Study on the possibilities of obtaining larva from Native Flat Oysters (Ostrea edulis L.) living in the Black Sea and larval metamorphosis stage. Turk] Zool, 24, 343- 350.
  • Vasconcelos GJ, Lee JS (1972). Microbial Flora of PaciŞc Oysters (Crassastrea gigas) Subjected to Ultraviolet-Irradiated Seawater. Appl Microbial, 23 (1), 11-16.
  • Venkateswaran K, Moser DP, Dollhopf ME, Lies DP, Saffarini DA, Macgregor BJ, Ringelberg DB, White DC, Nishij ima M, Sano H, Berghardt J, Stackebrandt E, Nealson, KH (1999). Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol, 49, 705-724.
  • Williams HR, Macey BM, Burnett LE, Burnett KG (2008). Differential localization and bacteriostasis of Vibrio campbelli among tissues of the Eastern oyster, Crassastrea virginica, 1-9.
There are 34 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Nilay Sütçüoğlu This is me

Jale Korun

Publication Date February 20, 2013
Submission Date February 20, 2013
Published in Issue Year 2011 Volume: 26 Issue: 2

Cite

APA Sütçüoğlu, N., & Korun, J. (2013). DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA. Aquatic Sciences and Engineering, 26(2), 13-26.
AMA Sütçüoğlu N, Korun J. DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA. Aqua Sci Eng. February 2013;26(2):13-26.
Chicago Sütçüoğlu, Nilay, and Jale Korun. “DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA”. Aquatic Sciences and Engineering 26, no. 2 (February 2013): 13-26.
EndNote Sütçüoğlu N, Korun J (February 1, 2013) DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA. Aquatic Sciences and Engineering 26 2 13–26.
IEEE N. Sütçüoğlu and J. Korun, “DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA”, Aqua Sci Eng, vol. 26, no. 2, pp. 13–26, 2013.
ISNAD Sütçüoğlu, Nilay - Korun, Jale. “DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA”. Aquatic Sciences and Engineering 26/2 (February 2013), 13-26.
JAMA Sütçüoğlu N, Korun J. DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA. Aqua Sci Eng. 2013;26:13–26.
MLA Sütçüoğlu, Nilay and Jale Korun. “DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA”. Aquatic Sciences and Engineering, vol. 26, no. 2, 2013, pp. 13-26.
Vancouver Sütçüoğlu N, Korun J. DETECTION OF NATURAL BACTERIAL BIOTA OF EUROPEAN FLAT OYSTER (OSTREA EDULIS, L. 1758) FROM NORTHERN SEA OF MARMARA. Aqua Sci Eng. 2013;26(2):13-26.

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